Glossary entry (derived from question below)
English term or phrase:
pour off
Polish translation:
odprowadzenie nadmiaru cieczy (z góry naczynia/zbiornika)
Added to glossary by
Frank Szmulowicz, Ph. D.
Mar 22, 2018 20:44
6 yrs ago
1 viewer *
English term
United Kingdom
Sworn, experienced, reliable
Native in: Polish
Works in: English to Polish, Polish to English, English, and 1 more.
pour off
English to Polish
Science
Environment & Ecology
general
Z tabeli NPL:
MPN is per ml of the 1 mL added to 9 mL rehydrated media (pour off is accounted for).
MPN is per ml of the 1 mL added to 9 mL rehydrated media (pour off is accounted for).
Proposed translations
(Polish)
| 2 | odprowadzenie nadmiaru cieczy (z góry naczynia/zbiornika) |
Frank Szmulowicz, Ph. D.
|
| 3 | zlać |
geopiet
|
References
| Test Procedure |
geopiet
|
Change log
Jun 21, 2018 20:08: Frank Szmulowicz, Ph. D. Created KOG entry
Proposed translations
3 hrs
Frank Szmulowicz, Ph. D.
United States
United States
Native in English
&
Polish
United States
On time, on target, on budget.
Native in: English 
, Polish
Works in: Polish to English, English to Polish, Russian to Polish, and 1 more.
Related KudoZ points
:
249
Selected
odprowadzenie nadmiaru cieczy (z góry naczynia/zbiornika)
to drain liquid off the top of something
https://idioms.thefreedictionary.com/pour off
https://idioms.thefreedictionary.com/pour off
4 KudoZ points awarded for this answer.
6 hrs
zlać
WYKONANIE ĆWICZENIA
1. Przygotować się do pracy pod komorą laminarną (patrz: ćwiczenie „Hodowle komórkowe”)
2. Wykonać pasaż komórek:
a) z naczynia hodowlanego zlać pożywkę
b) przepłukać komórki 1 ml PBSu
c) po zlaniu PBSu dodać do komórek 1 ml trypsyny i inkubować ok. 10 sekund
d) zlać nadmiar roztworu enzymu, pozostawiając jedynie jego niewielką ilość i umieścić naczynie w cieplarce na ok. 2-3 minuty, kontrolując („pod światło”) co jakiś czas stopień odrywania się komórek od podłoża
http://www.uj.edu.pl/documents/2387936/4117782/cw7bc.pdf - page 3
1. Przygotować się do pracy pod komorą laminarną (patrz: ćwiczenie „Hodowle komórkowe”)
2. Wykonać pasaż komórek:
a) z naczynia hodowlanego zlać pożywkę
b) przepłukać komórki 1 ml PBSu
c) po zlaniu PBSu dodać do komórek 1 ml trypsyny i inkubować ok. 10 sekund
d) zlać nadmiar roztworu enzymu, pozostawiając jedynie jego niewielką ilość i umieścić naczynie w cieplarce na ok. 2-3 minuty, kontrolując („pod światło”) co jakiś czas stopień odrywania się komórek od podłoża
http://www.uj.edu.pl/documents/2387936/4117782/cw7bc.pdf - page 3
Reference comments
6 hrs
Reference:
Test Procedure
1. Hydrate media by filling media vessel to the 100 mL mark with sterile diluent (e.g., dechlorinated water, deionized water, phosphate buffer, or 0.1% peptone), recapping and shaking to dissolve. See image #1.
2. Pipette 1 mL sample and then 9 mL of the rehydrated media onto the center of the plate base. See image #2.
3. Cover the plate with the lid and gently swirl to distribute the sample into all the wells. See image #3. NOTE: Air bubbles in the wells do not interfere with the test.
4. Tip the plate 90–120° to drain excess sample into the absorbent pad. See image #4.
5. Invert the plate and incubate for 48 hours at 35±0.5°C. See image #5.
6. Count the number of wells showing any fluorescence by holding a 6-watt, 365-nm UV light 5 inches above the plate. Direct light away from your eyes and towards the sample. Alternatively, you may read
fluorescent wells through the back of the inverted SimPlate base.
7. Refer to the MPN table provided to determine the Most Probable Number of heterotrophic plate count bacteria in the original sample. The table takes into account the sample/media poured off in step #4 above.
http://www.idexx.es/resource-library/water/simplate-for-hpc-...
2. Pipette 1 mL sample and then 9 mL of the rehydrated media onto the center of the plate base. See image #2.
3. Cover the plate with the lid and gently swirl to distribute the sample into all the wells. See image #3. NOTE: Air bubbles in the wells do not interfere with the test.
4. Tip the plate 90–120° to drain excess sample into the absorbent pad. See image #4.
5. Invert the plate and incubate for 48 hours at 35±0.5°C. See image #5.
6. Count the number of wells showing any fluorescence by holding a 6-watt, 365-nm UV light 5 inches above the plate. Direct light away from your eyes and towards the sample. Alternatively, you may read
fluorescent wells through the back of the inverted SimPlate base.
7. Refer to the MPN table provided to determine the Most Probable Number of heterotrophic plate count bacteria in the original sample. The table takes into account the sample/media poured off in step #4 above.
http://www.idexx.es/resource-library/water/simplate-for-hpc-...
Discussion
MPN - Most Probable Number