Glossary entry (derived from question below)
English term or phrase:
the reactions were performed
German translation:
die Reaktionen wurden durchgeführt
Added to glossary by
Klaus Urban
Sep 7, 2010 14:17
13 yrs ago
English term
the reactions were performed
English to German
Science
Biology (-tech,-chem,micro-)
Biotechnologie
Es geht um die DNA-Analyse der drei ältesten Kamelien Europas, um herauszufinden, ob sie alle derselben Sorte angehören.
"For the molecular marker analysis, DNA was isolated from 50 mg of leaves with EZNA HP Plant DNA Kit according to the manufacturer protocol (Omega Bio-Tek). A set of fourteen polymorphic microsatellites developed in C. japonica (Ueno et al., 1999; Abe et al., 2006) and C. sinensis (Freeman et al., 2004; Hung et al., 2008; Zhao et al., 2008), was tested (Table 2). DNA fragments were amplified using the tailed primer method (Shuelke, 2000). Polymorphisms of 14 microsatellite loci were assessed in a maximum of 95 plants from the living collection of Diputación Provincial de Pontevedra. ***The reactions were performed*** in 25 μL total volume containing 50 ng of genomic DNA."
"Das Ergebnis war ein Gesamtvolumen von 25 μL mit 50 ng genomischer DNA."
Habe ich das richtig verstanden?
"For the molecular marker analysis, DNA was isolated from 50 mg of leaves with EZNA HP Plant DNA Kit according to the manufacturer protocol (Omega Bio-Tek). A set of fourteen polymorphic microsatellites developed in C. japonica (Ueno et al., 1999; Abe et al., 2006) and C. sinensis (Freeman et al., 2004; Hung et al., 2008; Zhao et al., 2008), was tested (Table 2). DNA fragments were amplified using the tailed primer method (Shuelke, 2000). Polymorphisms of 14 microsatellite loci were assessed in a maximum of 95 plants from the living collection of Diputación Provincial de Pontevedra. ***The reactions were performed*** in 25 μL total volume containing 50 ng of genomic DNA."
"Das Ergebnis war ein Gesamtvolumen von 25 μL mit 50 ng genomischer DNA."
Habe ich das richtig verstanden?
Proposed translations
(German)
| 5 +5 | die Reaktionen wurden [...] durchgeführt |
Dr. Matthias Schauen
|
Change log
Sep 8, 2010 07:46: Steffen Walter changed "Field (specific)" from "Botany" to "Biology (-tech,-chem,micro-)"
Proposed translations
+5
3 mins
Dr. Matthias Schauen
Germany
Germany
Native in German
Specializes in field
Germany
Related KudoZ points
:
169
Selected
die Reaktionen wurden [...] durchgeführt
...wurden in einem Gesamtvolumen von 25 µl mit 50 ng genomischer DNA durchgeführt.
Hier wird das Reaktionsvolumen beschrieben, nicht das Ergebnis der Reaktion.
Hier wird das Reaktionsvolumen beschrieben, nicht das Ergebnis der Reaktion.
Note from asker:
| Danke!!!! |
4 KudoZ points awarded for this answer.
Comment: "Danke!"
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