English to Italian Law/Patents Biology (-tech,-chem,micro-) brevetto ossalato

Washes can be effectively accomplished by filling wells with multichannel pipettor.
For increased stringency, after each wash, let the plate stand briefly, *flick off* the buffer, and blot plates on paper towels before refilling.

e ancora

(3) Bring the plate to room temperature, *flick off* the capture antibody solution, wash 3 times with PBS-T buffer (1.5mM KH2PO4; 8.1mM Na2HPO4·12H2O; 136mM NaCl; 2.7mM KCl; 0.05% Tween-20), and block non-specific binding sites by adding 300µl of blocking solution(1.5mMKH2PO4; 8.1mM Na2HPO4.12H2O; 136mM NaCl; 2.7mM KCl; 5% non-fat dry milk) to each well.

grazie in anticipo