Diazyme Total Bile Acids Assay Kit is intended for the in vitro quantitative determination of total bile acids (TBA) in human serum. Total bile acids are metabolized in the liver and serve as a marker for normal and abnormal liver function. Serum total bile acids are increased in patients with liver disease.

Assay Principle
The reagents of the assay kit are in a stable liquid formulation that allows for ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α-hydroxysteroid dehydrogenase (3- α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α-HSD can convert 3-keto steroids and Thio- NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405 nm.

https://www.diazyme.com/total-bile-acids-enzyme-cycling-meth...

Galzuren

Techniek/methode

Diazyme Total Bile Acids Assay Kit
Enzymatisch, fotospectrometrisch (Enzyme Cycling Method, 5th generation)

https://www.azsintjan.be/nl/labogids/sint-jan-644/stofwissel...


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Note added at 1 hr (2020-06-24 14:40:10 GMT)
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Als je naar dit plaatje kijkt: https://www.researchgate.net/figure/Coupled-oxidoreductions-...

gaat het om een 'ronddraaiende enzymreactie'

galzuren worden omgezet in 3-ketosteroïden (althans in de test hierboven) en weer terug

Als je veel NADH toevoegt gaat het systeem effectief ronddraaien en wordt de vorming van Thio-NADH fotospectrometrisch gemeten