Spyros Salimpas (asker) Nov 28, 2015:

Conjugated bilirubin reacts with diazotised suplhanilic acid to produce an acid azobilirubin, the absorbance of which is proportional to the concentration of direct bilirubin in the sample and can be measured at 550 nm.

Anna Spanoudaki-Thurm Nov 28, 2015:

Είναι μόνο του ή έχει συγκείμενο;
Αν έχει, μπορείς να μας το δώσεις;