sterile 1 X PBS

French translation: tampon phosphate salin 1X stérile

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:sterile 1 X PBS
French translation:tampon phosphate salin 1X stérile
Entered by: Bertrand Leduc

15:59 Aug 12, 2013
English to French translations [PRO]
Tech/Engineering - Biology (-tech,-chem,micro-) / manip de labo
English term or phrase: sterile 1 X PBS
dissolve in 500 µl of sterile 1 X PBS

PBS = Phosphate Buffer Saline (tampon phosphate) mais c'est le 1 X qui me pose problème. S'agit-il de M (molaire) en fait ?
orgogozo
France
Local time: 02:05
tampon phosphate salin 1X stérile
Explanation:


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Note added at 27 mins (2013-08-12 16:27:25 GMT)
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1 X concentré une fois
5 X concentré cinq fois etc

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Note added at 28 mins (2013-08-12 16:28:42 GMT)
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http://fr.wikipedia.org/wiki/Tampon_TBE

TBE 10x : Solution mère concentrée dix fois, à diluer pour obtenir la concentration désirée.
TBE 0,5x : solution prête à l'emploi


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Note added at 31 mins (2013-08-12 16:31:08 GMT)
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page 3 de : www-dsv.cea.fr/var/plain/.../SERV_REF_002_b.doc‎


- Tampon de Laemmli 5X : mélanger 5mL de Tris / HCl 0.5M pH 6.8, 2g de SDS, 4g de glycérol, 5mL de β-mercaptoéthanol, 1.25mL de Bleu de bromophénol à 1%, qsp 20mL d’eau Ultrapure. Aliquoter le tampon par 1 mL et le conserver à -20°C pendant 1 an maximum.
Selected response from:

Bertrand Leduc
United Kingdom
Local time: 01:05
Grading comment
Merci à tous
4 KudoZ points were awarded for this answer



Summary of answers provided
4 +1tampon phosphate salin 1X stérile
Bertrand Leduc
4PBS stérile 1x
FX Fraipont (X)
3Solution prête à l'emploi, stérile, dans une solution de Tampon phosphate salin (PBS), pH 7.4
liz askew
Summary of reference entries provided
reference
liz askew

Discussion entries: 1





  

Answers


27 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
sterile 1 x pbs
PBS stérile 1x


Explanation:
à utiliser tel quel , sans dilution

"Solutions and dilutions: working with stock solutions - Rice University
http://www.ruf.rice.edu/~bioslabs/methods/solutions/stocks.h...
We define a stock solution as a concentrate, that is, a solution to be diluted to some lower ... A normal working solution is a 1x, or normal strength solution."


" Que veux dire le "X" dans les concentration
Cela veut dire que pour avoir la bonne concentration finale il faut diluer 20 fois ta solution 20X. Exemple: 10mL de solution A 20X + 190mL d'H2O = 200mL solution A 1X"
http://forums.futura-sciences.com/biologie/173023-veux-dire-...

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Note added at 29 mins (2013-08-12 16:29:15 GMT)
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"[Divers] URGENT Préparations PBS, la vraie? - Forum FS Generation ...
http://forums.futura-sciences.com/.../168344-urgent-prepara....‎Translate this page
Sep 24, 2007 - voilà j'utilise souvent du PBS pour les manips d'immunohisto. ... du tout fait. nous on achete du PBS 10X et on le dilue pour en avoir du 1X ..."

FX Fraipont (X)
Belgium
Local time: 02:05
Works in field
Native speaker of: Native in FrenchFrench
PRO pts in category: 135
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26 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +1
sterile 1 x pbs
tampon phosphate salin 1X stérile


Explanation:


--------------------------------------------------
Note added at 27 mins (2013-08-12 16:27:25 GMT)
--------------------------------------------------

1 X concentré une fois
5 X concentré cinq fois etc

--------------------------------------------------
Note added at 28 mins (2013-08-12 16:28:42 GMT)
--------------------------------------------------

http://fr.wikipedia.org/wiki/Tampon_TBE

TBE 10x : Solution mère concentrée dix fois, à diluer pour obtenir la concentration désirée.
TBE 0,5x : solution prête à l'emploi


--------------------------------------------------
Note added at 31 mins (2013-08-12 16:31:08 GMT)
--------------------------------------------------

page 3 de : www-dsv.cea.fr/var/plain/.../SERV_REF_002_b.doc‎


- Tampon de Laemmli 5X : mélanger 5mL de Tris / HCl 0.5M pH 6.8, 2g de SDS, 4g de glycérol, 5mL de β-mercaptoéthanol, 1.25mL de Bleu de bromophénol à 1%, qsp 20mL d’eau Ultrapure. Aliquoter le tampon par 1 mL et le conserver à -20°C pendant 1 an maximum.

Bertrand Leduc
United Kingdom
Local time: 01:05
Specializes in field
Native speaker of: Native in FrenchFrench
PRO pts in category: 184
Grading comment
Merci à tous

Peer comments on this answer (and responses from the answerer)
agree  GILLES MEUNIER
1 hr
  -> Merci Gilou !
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50 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
sterile 1 x pbs
Solution prête à l'emploi, stérile, dans une solution de Tampon phosphate salin (PBS), pH 7.4


Explanation:
,,

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Note added at 55 mins (2013-08-12 16:54:56 GMT)
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sorry

I think that should be

*****solution stérile de PBS prête à l'emploi******

Results for similar searches
Tampon phosphate salin - Wikipédia
fr.wikipedia.org/wiki/Tampon_phosphate_salin‎Translate this page
Pour préparer 1000 mL de PBS 1x : ... Ils sont formulés pour donner une solution de PBS prête à l'emploi après dissolution dans une certaine quantité d'eau ...

liz askew
United Kingdom
Local time: 01:05
Native speaker of: Native in EnglishEnglish
PRO pts in category: 24
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Reference comments


43 mins
Reference: reference

Reference information:
they seem to be referring to a 1X solution

PBS (Phosphate Buffered Saline) sterile solution pH 7.4 (1X ...
www.plantmedia.com/index.php?main_page=product_info...
Phosphate Buffered Saline, 1X Sterile - for cell culture and other research uses Technical Information: Ultra Pure Grade pH at 25°CC: 7.4+/- 0.1 DNase Activity: ...
PBS: 1 L of 10X | Santa Cruz Biotech
www.scbt.com › Chemicals › Prepared Buffers‎
Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X PBS buffer with a phosphate buffer concentration of ..

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Note added at 43 mins (2013-08-12 16:43:25 GMT)
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www.ruf.rice.edu/~bioslabs/methods/solutions/stocks.htm
We define a stock solution as a concentrate, that is, a solution to be diluted to some lower ... A normal working solution is a 1x, or normal strength solution.

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Note added at 45 mins (2013-08-12 16:45:15 GMT)
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MAKING SOLUTIONS
www.auburn.edu/~mathest/Files/labbasics.doc
Making 1X solutions: Stock solutions labeled as 5X (or other number) are 5 times the normal working strength of the solution. 100 ml of 5X stock and 400 ml DD ...

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Note added at 50 mins (2013-08-12 16:50:11 GMT)
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fr.cyclopaedia.net/wiki/Tampon-phosphate-salin‎Translate this page
Protocole. : Solution prête à l'emploi, stérile, dans une solution de Tampon phosphate salin (PBS), pH 7.4, aliquoté dans des tubes à usage unique, 220µl/tube.

liz askew
United Kingdom
Native speaker of: Native in EnglishEnglish
PRO pts in category: 24
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