English to Spanish Science Chemistry; Chem Sci/Eng biochemistry

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De antemano gracias.

Modifications to the Schreier method, e.g. by Brutschy (1984) included ethoxylation of 3-
hydroxy-carbofuran and a procedure for the isolation and determination of the phenol metabolites.
For the determination of phenol metabolites the hydrolysed and filtered sample was partitioned with
methylene chloride/diethyl ether (3:3), butylated hydroxytoluene (1 ml of 10 mg/kg in methylene
chloride) and ethanol were added to the extract and the solution was concentrated to remove
methylene chloride, acidified, and refluxed for 45 minutes. It was then partitioned into methylene
chloride, which was concentrated, cleaned up on a silica gel solid-phase extraction column
conditioned with methanol/water (1:1) and eluted with methylene chloride. The eluate was
concentrated and analysed by GLC on a cross-linked dimethyl silicone capillary column operated in
the splitless mode. Detection was mass-selective with monitoring of the molecular ions of the 7-
phenol, 3-keto-7-phenol and 3-ethoxy-7-phenol (164+., 178+. and 208+.). Calibration was with external
standards.