Spike/recovery experiments
Serbian translation: merenje (procenta) prinosa analitičkog postupka za poznatu dodatu količinu analita u uzorak
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| 16:08 Jan 8, 2013 |
| English to Serbian translations [PRO] Medical - Medical: Pharmaceuticals | |||||||
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|  Selected response from: LogosART Croatia Local time: 06:20 | ||||||
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| 4 | merenje (procenta) prinosa analitičkog postupka za poznatu dodatu količinu analita u uzorak |
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| Discussion entries: 1 | |
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Answers
4 hrs confidence: 
| spike/recovery experiments merenje (procenta) prinosa analitičkog postupka za poznatu dodatu količinu analita u uzorak Explanation: probe za merenje (procenta) prinosa analitičkog postupka (recovery) za poznatu dodatu (spike) količinu analita u uzorak http://www.doiserbia.nb.rs/img/doi/0367-598X/2002/0367-598X0... Cilj postupka je odrediti udio analita u uzorku, uzimajući u obzir iskorištenje (engl. recovery) analitičkog postupka. Spike 2. Promišljeno dodavanje poznate količine poznate supstanceanalitičkom uzorku, korišćenom za vrednovanje analitičke tehnike. Spike and Recovery A. The Meaning and Purpose of Spike-and-Recovery Assessment In spike-and-recovery, a known amount of analyte is added (spiked) into the natural test sample matrix and its response is measured (recovered) in the assay by comparison to an identical spike in the standard diluent. ELISA methods involve comparison of test samples to a standard curve prepared using known concentrations of the analyte (e.g., purified recombinant protein). The goal in assay development is to maximize signal-to-noise ratio while achieving identical responses for a given amount of analyte in standard diluent (the standard curve) and sample matrix (biological sample + sample diluent). The sample matrix may contain components that affect assay response to the analyte differently than the standard diluent. A spike-and-recovery experiment is designed to assess this difference in assay response. Recovery je mera mogućnosti analitičke metode da tačno izmeri čistu supstancu kada se njena poznata količina (spike) doda u uzorak koji se rutinski analizira, pri čemu se uzima u obzir uticaj svih supstanci realno prisutnih u matriksu tog uzorka. Zbog toga je recovery u direktnoj vezi sa tačnošću metode i može da se koristi za procenu njene primenljivosti, naročito u situacijama kada nema odgovarajuće referentne metode ili referentnog materijala. Vrednosti rezultata oporavka između 95 i 105 % predstavljaju dokaz odsustva interferencija u određivanju analita. Kada se proverava recovery, problem predstavljaju često osobine same supstance (fizičko stanje u kome se nalazi, rastvorljivost itd.), a nekad i nemogućnost da se odgovarajuća supstanca dobije. http://www.pmf.ni.ac.rs/pmf/doktorati/doc/27_06_2011_r_i.pdf surogat-uzorci (spike recovery) • slepe probe • analiza standardnih uzoraka • surogat-uzorci (spike recovery) • kontrolni uzorci • ponovljena merenja • kontrolni dijagrami (karte) http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd... |
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