English to French Medical Medical (general)

EAE was induced with 200 micrograms of PLP (proeolipid protein) 139–151, PLP 178–191 or MBP (myelin basic protein) 87–106, emulsified in IFA supplemented with 200 micrograms of M y c o b a c t e r i u m tuberculosis and M. butyricum 8:1 and given subcutaneously (s.c.) on days 0 and 7. Immediately after this "immunisation" and 48 hours later, mice received 200 nanograms of Bordetella pertussis toxin (intraperitoneally) in PBS (protein baseline serum).
Without going into great detail, strong proliferation to the PLP 178–191 peptide used to induce disease was detected as early as day 4 after "immunisation", reaching a peak by days 9–11. At the time of remission, days 15–16, a considerable decrease in proliferative capacity of lymph node cells was detected.IFN-gamma (interferon gamma) production followed the same pattern; some variability was observed between individual mice, but a relatively high concentration was measured during the first 11 days, decreasing thereafter.

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