PCR individualmente para cada par de primer

English translation: each primer pair was individually submitted to PCR.

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Portuguese term or phrase:	PCR individualmente para cada par de primer
English translation:	each primer pair was individually submitted to PCR.
Entered by:	Christina Paiva

20:50 Apr 11, 2011

Portuguese to English translations [PRO]
Genetics

Portuguese term or phrase: PCR individualmente para cada par de primer

O DNA genômico foi extraído a partir de 3 x 106 células utilizando o kit DNAzol (Invitrogen®), conforme protocolo proposto pelo fabricante, seguido pela PCR individualmente para cada par de primer aroA e dpr utilizando 200 µM dNTP, 3,4 mM MgCl2, 2 U de Taq DNA Polimerase (Invitrogen®), 1,6x tampão de amplificação e 0,20 μM dos primers em um volume final de 25 µL.

Andre Damasceno KudoZ activity Questions: 4718 (8 open) (115 closed without grading) Answers: 780 Brazil
Local time: 00:57

each primer pair was individually submitted to PCR.

Explanation:
Sugestão:

by the manufacturer. Subsequently, each...

Selected response from:

Christina Paiva
Brazil
Local time: 00:57

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Summary of answers provided

followed by PCR individually for each primer pair

Walter Moura

each primer pair was individually submitted to PCR.

Christina Paiva

Answers

3 hrs confidence:

followed by PCR individually for each primer pair

Explanation:
http://en.wikipedia.org/wiki/Polymerase_chain_reaction

The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Multiplex Ligation-dependent Probe Amplification (MLPA): permits multiple targets to be amplified with only a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below).

http://www.med.nyu.edu/rcr/rcr/course/primer.html

*** For PCR primer pair selection, you can choose a target range of the template sequence to be amplified.

Walter Moura
Brazil
Local time: 00:57
Native speaker of:

Portuguese
PRO pts in category: 6

8 hrs confidence:

each primer pair was individually submitted to PCR.

Explanation:
Sugestão:

by the manufacturer. Subsequently, each...

Christina Paiva
Brazil
Local time: 00:57
Specializes in field
Native speaker of:

Portuguese,

English
PRO pts in category: 12

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PCR individualmente para cada par de primer

English translation: each primer pair was individually submitted to PCR.

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PCR individualmente para cada par de primer

English translation: each primer pair was individually submitted to PCR.

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