Durchfixierung

English translation: complete fixation

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
German term or phrase:Durchfixierung
English translation:complete fixation
Entered by: Rowan Morrell

05:59 Sep 20, 2012
German to English translations [PRO]
Medical - Medical: Pharmaceuticals / Laboratory Experiments on Rats
German term or phrase: Durchfixierung
"Histologische Untersuchung bei Versuchsende
Nach Durchfixierung wurden die Organe zurechtgeschnitten oder Proben entnommen und im Entwässerungsautomaten weiterbearbeitet."

From a text about an experiment on lab rats. This is describing what happens after they're put down. The text has previously mentioned about organs being "fixiert" in formalin, which I guess is just fixed, but I have no idea what "Durchfixierung" is and can't find a translation for it (or the verb "durchfixieren" anywhere). Google searching for "fixed through" in relation to animal organs in experiments is utterly inconclusive. TIA for any light you can shed on this decidedly obscure word.
Rowan Morrell
New Zealand
Local time: 10:01
complete fixation
Explanation:
Once fixation is complete...

Effects of Prolonged Formalin Fixation on Diagnostic ...
www.ncbi.nlm.nih.gov › ... › J Histochem Cytochem › v.57(8); Aug 2009
by JD Webster - 2009 - Cited by 13 - Related articles
It takes ~25 hr for formalin to penetrate a 1-cm3 sphere of tissue (Eltoum et al. 2001b). If tissues are processed before complete fixation, unfixed tissue will be ...
[PDF]

Fixation and Fixatives (4) – Popular Fixative Solutions: Pathology ...
www.leica-microsystems.com/.../fixation-and-fixatives-4-pop...
6 Mar 2012 – Combines a denaturing fixative with the additive and cross-linking effects of formalin. Is sometimes used during processing to complete fixation ...

Guidelines for Transport, Handling, and Fixation of Tissues
pathology.wustl.edu/research/cores/amp/index.php?page...
After complete fixation in formalin, tissues can be safely transferred to 70% ethanol at 4o C until processing. As implied above, under-fixation should be avoided.
Selected response from:

SJLD
Local time: 00:01
Grading comment
Complete fixation it is! Thanks for a thorough and convincing answer SJLD, and thanks to the peer graders for making my decision that much easier. But thanks also to Zareh for setting me straight about fixation. Appreciate everyone's help.
4 KudoZ points were awarded for this answer



Summary of answers provided
4 +5complete fixation
SJLD
2immersion fixation
Zareh Darakjian Ph.D.


Discussion entries: 3





  

Answers


13 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +5
complete fixation


Explanation:
Once fixation is complete...

Effects of Prolonged Formalin Fixation on Diagnostic ...
www.ncbi.nlm.nih.gov › ... › J Histochem Cytochem › v.57(8); Aug 2009
by JD Webster - 2009 - Cited by 13 - Related articles
It takes ~25 hr for formalin to penetrate a 1-cm3 sphere of tissue (Eltoum et al. 2001b). If tissues are processed before complete fixation, unfixed tissue will be ...
[PDF]

Fixation and Fixatives (4) – Popular Fixative Solutions: Pathology ...
www.leica-microsystems.com/.../fixation-and-fixatives-4-pop...
6 Mar 2012 – Combines a denaturing fixative with the additive and cross-linking effects of formalin. Is sometimes used during processing to complete fixation ...

Guidelines for Transport, Handling, and Fixation of Tissues
pathology.wustl.edu/research/cores/amp/index.php?page...
After complete fixation in formalin, tissues can be safely transferred to 70% ethanol at 4o C until processing. As implied above, under-fixation should be avoided.

SJLD
Local time: 00:01
Meets criteria
Specializes in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 30
Grading comment
Complete fixation it is! Thanks for a thorough and convincing answer SJLD, and thanks to the peer graders for making my decision that much easier. But thanks also to Zareh for setting me straight about fixation. Appreciate everyone's help.

Peer comments on this answer (and responses from the answerer)
agree  Zareh Darakjian Ph.D.: It would seem to me that this is the correct translation for the term in question.
44 mins
  -> thanks Dr Z! :-)

agree  Dr Lofthouse
1 hr

agree  Anne Schulz: complete as "in toto", I suppose (since rat organs are small enough to fit into a cassette)
9 hrs

agree  uyuni
10 hrs

agree  Harald Moelzer (medical-translator)
1 day 58 mins
Login to enter a peer comment (or grade)

21 mins   confidence: Answerer confidence 2/5Answerer confidence 2/5
immersion fixation


Explanation:
This could be "immersion fixation", the other two major types being

"heat fixation" and "immersion".

http://en.wikipedia.org/wiki/Fixation_(histology)#Types_of_f...

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Note added at 23 mins (2012-09-20 06:22:42 GMT)
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http://en.wikipedia.org/wiki/Fixation_(histology)

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Note added at 24 mins (2012-09-20 06:23:33 GMT)
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**Immersion**: The sample of tissue is immersed in fixative of volume at a minimum of 20 times greater than the volume of the tissue to be fixed. The fixative must diffuse through the tissue to fix, so tissue size and density, as well as type of fixative must be considered. Using a larger sample means it takes longer for the fixative to reach the deeper tissue. Best in a slight vacuum.

Zareh Darakjian Ph.D.
United States
Local time: 15:01
Meets criteria
Specializes in field
Native speaker of: Native in EnglishEnglish, Native in ArmenianArmenian
PRO pts in category: 112
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