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  3. Medical: Pharmaceuticals

BLOCAGE ET FIXATION

English translation: blocking and binding

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
French term or phrase:BLOCAGE ET FIXATION
English translation:blocking and binding
Entered by: Bashiqa

07:41 Sep 14, 2020
French to English translations [PRO]
Medical - Medical: Pharmaceuticals / sample preparation
French term or phrase: BLOCAGE ET FIXATION
Context:
ELECTRO-TRANSFERT, BLOCAGE ET FIXATION DES ANTICORPS SUR LES
MEMBRANES POUR LA METHODE WET

What is the difference between the two terms, or is it a literal translation?
TIA Chris.
Bashiqa
France
Local time: 00:53
blocking and binding
Explanation:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3456489/

As the antibodies only bind to the protein of interest, only one band should be visible. The thickness of the band corresponds to the amount of protein present; thus doing a standard can indicate the amount of protein present.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:16:04 GMT)
--------------------------------------------------

I am not clear which technique is being used here, Western Blotting?

However it is clearly an "immuno" technique (eg immunohistochemistry) involving the use of antibodies selective for specific proteins, involving the binding of these Abs to these proteins and their subsequent visualisation by various means. The blocking process involves prior application of a solution to prevent the antibodies binding non-specifically to the wrong proteins.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:17:25 GMT)
--------------------------------------------------

tglab.com/support/immunohistochemistry-protocol/ihc-blocking/#:~:text=What%20is%20blocking%20in%20Immunohistochemistry,specific%20binding%20to%20other%20molecules.

What is blocking in Immunohistochemistry?
Blocking is essential for preventing non-specific binding of antibodies or other reagents to the tissue. Even if the antibody has high specificity towards the target, intermolecular forces can promote non-specific binding to other molecules. Consequently, non-specific binding prevents visualization of the antigen-antibody binding of interest. To mitigate nonspecific binding, a blocking step should be carried out before incubation with the primary antibody.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:21:37 GMT)
--------------------------------------------------

https://en.wikipedia.org/wiki/Immunohistochemistry

Immunohistochemistry (IHC) is the most common application of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues

--------------------------------------------------
Note added at 10 hrs (2020-09-14 17:56:26 GMT)
--------------------------------------------------

https://www.sciencedirect.com/topics/medicine-and-dentistry/...

The separated protein is then transferred onto the membrane [polyvinylidene difluoride (PVDF) or nitrocellulose], where they bind to antibodies specific to the target proteins. Western blotting can detect target proteins as low as 1 ng in concentration due to high-resolution capacity of gel electrophoresis and strong sensitivity and specificity of the immunoassay.

--------------------------------------------------
Note added at 10 hrs (2020-09-14 18:07:29 GMT)
--------------------------------------------------

finally binding is specified as a translation of fixation on Terrmium
https://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-fra.html?l...

ubject field(s)
Nervous System
Biochemistry
binding
correct
CONT
Neurotransmitter receptors are proteins that span the neuronal membrane. These proteins have ligand binding regions that are accessible to extracellular messengers and other regions involved in transducing the binding interaction into an intercellular effect. The reversible binding of the neurotransmitter to the receptor causes a conformational change that triggers the transmembrane signaling event.

--------------------------------------------------
Note added at 10 hrs (2020-09-14 18:08:48 GMT)
--------------------------------------------------

see fiche 2 in Termium
Selected response from:

Sue Davis
France
Local time: 00:53
Grading comment
Thank you.
4 KudoZ points were awarded for this answer



Summary of answers provided
4blocking and binding
Sue Davis
4 -1Blocking and fixin
Michael Roberts
3blocking and fixation
liz askew


Discussion entries: 6





  

Answers


1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): -1
Blocking and fixin


Explanation:
Le plus simple est souvent le plus efficace

Michael Roberts
Morocco
Local time: 23:53
Specializes in field
Native speaker of: Native in EnglishEnglish, Native in FrenchFrench

Peer comments on this answer (and responses from the answerer)
neutral  writeaway: convincing refs
26 mins
disagree  Sue Davis: not correct in this context
9 hrs
Login to enter a peer comment (or grade)

2 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
blocking and fixation


Explanation:
https://www.abcam.com/protocols/immunocytochemistry-immunofl...

--------------------------------------------------
Note added at 2 hrs (2020-09-14 09:49:41 GMT)
--------------------------------------------------


Preparing Fixed Cells for Labeling | Thermo Fisher Scientific ...www.thermofisher.com › home › sample-considerations
Fixing cells has its advantages (and disadvantages) in fluorescence imaging. ... block your cells and then stain them with fluorescent dyes and/or antibody ... Cold alcohol fixation is sometimes recommended for membrane-surface antigens.

Flow Cytometry Protocol for Staining Membrane-associated ...www.rndsystems.com › Flow Cytometry Protocols
Reagents Required. Fc Receptor Blocking Reagents (These include Fc receptor blocking antibodies or IgG solutions) ...

liz askew
United Kingdom
Local time: 23:53
Works in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 465
Login to enter a peer comment (or grade)

2 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
blocking and binding


Explanation:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3456489/

As the antibodies only bind to the protein of interest, only one band should be visible. The thickness of the band corresponds to the amount of protein present; thus doing a standard can indicate the amount of protein present.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:16:04 GMT)
--------------------------------------------------

I am not clear which technique is being used here, Western Blotting?

However it is clearly an "immuno" technique (eg immunohistochemistry) involving the use of antibodies selective for specific proteins, involving the binding of these Abs to these proteins and their subsequent visualisation by various means. The blocking process involves prior application of a solution to prevent the antibodies binding non-specifically to the wrong proteins.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:17:25 GMT)
--------------------------------------------------

tglab.com/support/immunohistochemistry-protocol/ihc-blocking/#:~:text=What%20is%20blocking%20in%20Immunohistochemistry,specific%20binding%20to%20other%20molecules.

What is blocking in Immunohistochemistry?
Blocking is essential for preventing non-specific binding of antibodies or other reagents to the tissue. Even if the antibody has high specificity towards the target, intermolecular forces can promote non-specific binding to other molecules. Consequently, non-specific binding prevents visualization of the antigen-antibody binding of interest. To mitigate nonspecific binding, a blocking step should be carried out before incubation with the primary antibody.

--------------------------------------------------
Note added at 2 hrs (2020-09-14 10:21:37 GMT)
--------------------------------------------------

https://en.wikipedia.org/wiki/Immunohistochemistry

Immunohistochemistry (IHC) is the most common application of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues

--------------------------------------------------
Note added at 10 hrs (2020-09-14 17:56:26 GMT)
--------------------------------------------------

https://www.sciencedirect.com/topics/medicine-and-dentistry/...

The separated protein is then transferred onto the membrane [polyvinylidene difluoride (PVDF) or nitrocellulose], where they bind to antibodies specific to the target proteins. Western blotting can detect target proteins as low as 1 ng in concentration due to high-resolution capacity of gel electrophoresis and strong sensitivity and specificity of the immunoassay.

--------------------------------------------------
Note added at 10 hrs (2020-09-14 18:07:29 GMT)
--------------------------------------------------

finally binding is specified as a translation of fixation on Terrmium
https://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-fra.html?l...

ubject field(s)
Nervous System
Biochemistry
binding
correct
CONT
Neurotransmitter receptors are proteins that span the neuronal membrane. These proteins have ligand binding regions that are accessible to extracellular messengers and other regions involved in transducing the binding interaction into an intercellular effect. The reversible binding of the neurotransmitter to the receptor causes a conformational change that triggers the transmembrane signaling event.

--------------------------------------------------
Note added at 10 hrs (2020-09-14 18:08:48 GMT)
--------------------------------------------------

see fiche 2 in Termium

Sue Davis
France
Local time: 00:53
Specializes in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 68
Grading comment
Thank you.
Login to enter a peer comment (or grade)



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