GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW) | ||||||
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17:23 Oct 31, 2005 |
English language (monolingual) [PRO] Medical - Genetics | |||||
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| Selected response from: Veronica Prpic Uhing United States Local time: 15:00 | ||||
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SUMMARY OF ALL EXPLANATIONS PROVIDED | ||||
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5 +4 | SSC" is sodium chloride and sodium citrate solution |
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5 +1 | five times of suggested working concentration of SSC |
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Discussion entries: 4 | |
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5x ssc five times of suggested working concentration of SSC Explanation: . -------------------------------------------------- Note added at 55 mins (2005-10-31 18:18:45 GMT) -------------------------------------------------- Yes, SSC is an abbreviation, no need to translate it at all. Yes, what in parenthesis explain the word just before it. I think it's easy to understand this, so I did not said it. Sorry. (What I explained is the key word that may confuse people.) -------------------------------------------------- Note added at 6 hrs 58 mins (2005-11-01 00:21:47 GMT) Post-grading -------------------------------------------------- SSC is the abbreviation of "Solution of Sodium Citrate". |
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5x ssc SSC" is sodium chloride and sodium citrate solution Explanation: SSC is a abbreviation of sodium chloride and sodium citrate solution 5X SSC (0.75M NaCl, 0.075M sodium citrate) -------------------------------------------------- Note added at 6 mins (2005-10-31 17:29:25 GMT) -------------------------------------------------- 5x means it is 5 x concentrated and hence, needs to be diluted 5x in working buffer SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 10*SSC or 5*SSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis. http://www.protocol-online.org/archive/posts/3129.html -------------------------------------------------- Note added at 58 mins (2005-10-31 18:21:36 GMT) -------------------------------------------------- Note added at 6 mins (2005-10-31 17:29:25 GMT) -------------------------------------------------- 5x means it is 5 x concentrated and hence, needs to be diluted 5x into the working buffer -------------------------------------------------- Note added at 5 hrs 26 mins (2005-10-31 22:49:55 GMT) -------------------------------------------------- To XiaoRan: The real issue of importance here is the final concentrations of constituents in the assay. How you get there needs to be clarified ¨C 0.5 x of what, 2x of what, concentration of 5x (see above), especially when you decide to publish your data and communicate them to the others to understand and therefore better appreciate your work. -------------------------------------------------- Note added at 7 hrs 10 mins (2005-11-01 00:33:34 GMT) -------------------------------------------------- To XiaoRan: In this instance SSC is specified as: 5X SSC (0.75M NaCl, 0.075M sodium citrate), |
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