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TAP-tagged

Italian translation: proteine sinaptiche con TAP-tag

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:TAP-tagged
Italian translation:proteine sinaptiche con TAP-tag
Entered by: PLR TRADUZIO (X)

08:13 Dec 17, 2013
English to Italian translations [PRO]
Medical - Biology (-tech,-chem,micro-) / disturbi del sistema nervoso
English term or phrase: TAP-tagged
Un progetto di ricerca per cercare di associare le sinaptopatie alle mutazioni genetiche:

Behavioural and physiological correlates of the mutations have been described, and the underlying mechanisms have been analysed at the molecular and network level with the aim of developing novel diagnostic tools and therapies.(...)
Scientists completed large scale screens of protein-protein interactions (PPIs) of 1340 disease-relevant synaptic proteins and generated PPI networks of 7677 interactions.
Moreover, mouse lines were created that mimic disease-relevant mutations or express ***tandem affinity purification (TAP)-tagged ***synaptic proteins of disease-relevance for several applications such as protein purification and screening of substrates.


Grazie!!!
federica gagliardi
Italy
Local time: 03:27
proteine sinaptiche con TAP-tag
Explanation:
http://www.fedoa.unina.it/1691/1/Franci_Biochimica.pdf pagine 6 e 7:

Le prime applicazioni sperimentali del sistema TAP hanno riguardato la purificazione di complessi proteici nel lievito S.cerevisae (8), per poi essere trasferite anche in sistemi di mammiferi (9). Tale metodica prevede l’utilizzo della TAP-tag che conta 160 amminoacidi ed è costituita da due domini proteici, uno rappresentato dalla proteina A(protA) di S.Aureus e l’altro dalla proteina che lega la calmodulina (CBP), separati da un sito di taglio per la proteasi del virus del mosaico del tabacco (TEV) (Figura 2A).


http://en.wikipedia.org/wiki/Tandem_Affinity_Purification
Selected response from:

PLR TRADUZIO (X)
Italy
Local time: 03:27
Grading comment
grazie
4 KudoZ points were awarded for this answer



Summary of answers provided
4 +1proteine sinaptiche con TAP-tag
PLR TRADUZIO (X)
3sottoposte a purificazione mediante TAP-tag
Federica Borgini


  

Answers


19 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
tap-tagged
sottoposte a purificazione mediante TAP-tag


Explanation:
proteine sinaptiche sottoposte a purificazione mediante TAP-tag (tandem affinity purification)
forse non è ancora la soluzione perfetta ma spero possa aiutarti


    Reference: http://www.fedoa.unina.it/1691/1/Franci_Biochimica.pdf
Federica Borgini
Italy
Local time: 03:27
Works in field
Native speaker of: Italian
PRO pts in category: 39

Peer comments on this answer (and responses from the answerer)
neutral  PLR TRADUZIO (X): Non è quello che è scritto Federica, è scritto che proteine con TAP-tag sono espresse in mouse lines FOR several applications such as protein purification and screening of substrates.
2 hrs
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2 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +1
tap-tagged
proteine sinaptiche con TAP-tag


Explanation:
http://www.fedoa.unina.it/1691/1/Franci_Biochimica.pdf pagine 6 e 7:

Le prime applicazioni sperimentali del sistema TAP hanno riguardato la purificazione di complessi proteici nel lievito S.cerevisae (8), per poi essere trasferite anche in sistemi di mammiferi (9). Tale metodica prevede l’utilizzo della TAP-tag che conta 160 amminoacidi ed è costituita da due domini proteici, uno rappresentato dalla proteina A(protA) di S.Aureus e l’altro dalla proteina che lega la calmodulina (CBP), separati da un sito di taglio per la proteasi del virus del mosaico del tabacco (TEV) (Figura 2A).


http://en.wikipedia.org/wiki/Tandem_Affinity_Purification


PLR TRADUZIO (X)
Italy
Local time: 03:27
Specializes in field
Native speaker of: Native in ItalianItalian, Native in FrenchFrench
PRO pts in category: 108
Grading comment
grazie

Peer comments on this answer (and responses from the answerer)
agree  Federica Borgini: credo che tu abbia ragione
23 hrs
  -> Grazie Federica
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