stringent wash

08:53 Jun 25, 2010
English to Croatian translations [PRO]
Medical - Medical (general)
English term or phrase: stringent wash
detection of amplified HPV DNA using a manual procedure - stringent wash
Local time: 07:28

Summary of reference entries provided
stringent wash
Mira Stepanovic


Reference comments

1 day 2 hrs
Reference: stringent wash

Reference information:
second wash (the stringent wash)........., in 0.1-2x SSC, usually three times 5 minutes each. This step is used primarily to remove the non-specific and/or repetitive DNA hybridized to the cells and chromosomes. The less concentrated the salt solution and the longer the duration of the wash and the temperature, the higher the stringency and the more DNA will be removed. This wash can be done at temperatures between 25 and 75 C, in two-three steps, 2-5 minutes each. It is impossible to present a standard procedure, but there are some guidelines that can help. For very short DNA probes (0.5-3 kb) or very complex probes (chromosome paint probes, CGH), the washing temperature should be lower (up to 45 C) and the stringency lower as well (1x-2x SSC). When using single-locus, large probes (cosmids, BACs, P1s, YACs), the temperature should be around 65 C and the stringency high (below 0.5xSSC). The temperature and stringency should be highest for repetitive probes (such as alpha-satellite repeats).

Note added at 2 days1 hr (2010-06-27 10:20:07 GMT)

Izvinite molim vas, tek sada sam videla da sam navela pogrešan link, navedeni tekst sam našla ovde:
Nažalost, za sada nemam predlog prevoda.

Mira Stepanovic
Specializes in field
Native speaker of: Native in SerbianSerbian, Native in Serbo-CroatSerbo-Croat
PRO pts in category: 46
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