matrix spiked

Croatian translation: matrica/matriks obogaćen(a) (čime)

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:matrix spiked
Croatian translation:matrica/matriks obogaćen(a) (čime)
Entered by: V&E-Team

12:05 Oct 19, 2016
English to Croatian translations [PRO]
Medical - Genetics / PCR real-time test
English term or phrase: matrix spiked
glagol - spike (ed)
http://www.firstenv.com/definitions.htm


LABORATORY CONTROL SPIKE: A known matrix spiked with compound(s) representative of the target analytes. This is used to show that sample preparation procedures do not contribute to loss of analytes.

MATRIX SPIKE: An aliquot of sample spiked with a known concentration of target analyte(s). The spiking occurs prior to sample preparation and analysis. A matrix spike is used to document the bias of a method in a given sample matrix.

MATRIX SPIKE DUPLICATES: Intralaboratory split samples spiked with identical concentrations of target analyte(s). The spiking occurs prior to sample preparation and analysis. They are used to document the precision and bias of a method in a given sample matrix.
V&E-Team
Croatia
Local time: 20:41
matrica/matriks obogaćen(a) (čime)
Explanation:

Validacijsko ispitivanje bioanalitičkih postupaka uključuje odreñivanje u obogaćenim uzorcima («spiked» samples) pripravljenim iz referentnih standarda i biološkog matriksa te odreñivanjem relevantnih parametara koji opisuju izvedbu metode (1). Valja procijeniti ispravnost, preciznost, funkciju odgovora i selektivnost metode na endogene supstancije, metabolite i razgradne produkte u biološkom matriksu (27).

Obogaćeni/standardni/QC uzorci (1, 27). - Unutar validacijskog postupka koristi se isti biološki matriks za pripravu obogaćenih uzoraka, kalibracijskih standarda i uzoraka kontrole kvalitete («quality control», QC), koji se svi pripravljaju iz odvojenih odvaga. Svi se uzorci obrañuju na isti način. Biološki matriks treba takoñer validirati te navesti korišteni antikoagulans ili anestetik za dobivanje kontrolnog matriksa, materijal posuña, etc.

Obogaćeni uzorci («spiked samples», to su slijepi matriksi ili stvarni uzorci obogaćeni analitom) pripravljaju se iz kalibracijskih standarda (uzorci poznate čistoće, stabilnosti i uvjeta čuvanja: pripravljeni svježi svaki dan u istom matriksu).

cu, cs – koncentracija analita u uzorku, standardu; Vu, Vs – volumen uzorka, standarda;
Ru, Rs – analitički signal za razrijeñeni uzorak i obogaćeni «spiked» uzorak

SOURCE:
Sveučilište u Zagrebu Farmaceutsko-biokemijski fakultet
ODABRANE TEME IZ BIOANALITIKE
4. izdanje
Svjetlana Luterotti Dane Bicanic
Zagreb, 2013.
http://webcache.googleusercontent.com/search?q=cache:bqeHQL1...

Ako prirodno kontaminirani materijal nije na raspolaganju, treba upotrijebiti matriks obogaćen s analitom,…
http://narodne-novine.nn.hr/clanci/sluzbeni/287539.html

_____________

Matrix Spike (Spiked Sample or Fortified Sample)– A sample prepared by adding a known mass of target analyte to a specified amount of matrix sample for which an independent estimate of target analyte concentration is available. Matrix spikes are used, for example, to determine the effect of the matrix on a method's recovery efficiency. (ORD) (OW/EAD) (OPP) (OAR/OAQPS) (ORCR)

Matrix Spike (MS) and Matrix Spike Duplicate (MSD) – Two aliquots of the same environmental sample to which known quantities of the target analytes are added in the laboratory. The MS and MSD are analyzed exactly like a sample, and their purpose is: to determine whether the sample matrix contributes bias to the analytical results, and to indicate precision associated with laboratory procedures. The background concentrations of the analytes in the sample matrix must be determined in a separate aliquot and the measured values in the MS and MSD corrected for background concentrations. (ORD)

SOURCE:
Environmental Measurement
Glossary of Terms January 11, 2010
Selected response from:

LogosART
Croatia
Local time: 20:41
Grading comment
Hvala!
4 KudoZ points were awarded for this answer



Summary of answers provided
4matrica/matriks obogaćen(a) (čime)
LogosART
Summary of reference entries provided
matrica s dodanim standardom
ipv

  

Answers


9 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
matrica/matriks obogaćen(a) (čime)


Explanation:

Validacijsko ispitivanje bioanalitičkih postupaka uključuje odreñivanje u obogaćenim uzorcima («spiked» samples) pripravljenim iz referentnih standarda i biološkog matriksa te odreñivanjem relevantnih parametara koji opisuju izvedbu metode (1). Valja procijeniti ispravnost, preciznost, funkciju odgovora i selektivnost metode na endogene supstancije, metabolite i razgradne produkte u biološkom matriksu (27).

Obogaćeni/standardni/QC uzorci (1, 27). - Unutar validacijskog postupka koristi se isti biološki matriks za pripravu obogaćenih uzoraka, kalibracijskih standarda i uzoraka kontrole kvalitete («quality control», QC), koji se svi pripravljaju iz odvojenih odvaga. Svi se uzorci obrañuju na isti način. Biološki matriks treba takoñer validirati te navesti korišteni antikoagulans ili anestetik za dobivanje kontrolnog matriksa, materijal posuña, etc.

Obogaćeni uzorci («spiked samples», to su slijepi matriksi ili stvarni uzorci obogaćeni analitom) pripravljaju se iz kalibracijskih standarda (uzorci poznate čistoće, stabilnosti i uvjeta čuvanja: pripravljeni svježi svaki dan u istom matriksu).

cu, cs – koncentracija analita u uzorku, standardu; Vu, Vs – volumen uzorka, standarda;
Ru, Rs – analitički signal za razrijeñeni uzorak i obogaćeni «spiked» uzorak

SOURCE:
Sveučilište u Zagrebu Farmaceutsko-biokemijski fakultet
ODABRANE TEME IZ BIOANALITIKE
4. izdanje
Svjetlana Luterotti Dane Bicanic
Zagreb, 2013.
http://webcache.googleusercontent.com/search?q=cache:bqeHQL1...

Ako prirodno kontaminirani materijal nije na raspolaganju, treba upotrijebiti matriks obogaćen s analitom,…
http://narodne-novine.nn.hr/clanci/sluzbeni/287539.html

_____________

Matrix Spike (Spiked Sample or Fortified Sample)– A sample prepared by adding a known mass of target analyte to a specified amount of matrix sample for which an independent estimate of target analyte concentration is available. Matrix spikes are used, for example, to determine the effect of the matrix on a method's recovery efficiency. (ORD) (OW/EAD) (OPP) (OAR/OAQPS) (ORCR)

Matrix Spike (MS) and Matrix Spike Duplicate (MSD) – Two aliquots of the same environmental sample to which known quantities of the target analytes are added in the laboratory. The MS and MSD are analyzed exactly like a sample, and their purpose is: to determine whether the sample matrix contributes bias to the analytical results, and to indicate precision associated with laboratory procedures. The background concentrations of the analytes in the sample matrix must be determined in a separate aliquot and the measured values in the MS and MSD corrected for background concentrations. (ORD)

SOURCE:
Environmental Measurement
Glossary of Terms January 11, 2010


LogosART
Croatia
Local time: 20:41
Native speaker of: Dutch
PRO pts in category: 4
Grading comment
Hvala!
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Reference comments


15 mins
Reference: matrica s dodanim standardom

Reference information:
6.4.2. Regular blank controls and spiking experiments or analysis of control samples
6.4.2 Redovite slijepe kontrole i eksperimenti s dodanim standardom
http://eur-lex.europa.eu/legal-content/HR/TXT/?uri=celex:320...

i još dosta primjera:
metoda dodatka standarda. → složena matrica
https://www.pmf.unizg.hr/_download/repository/AK2_seminar_6_...



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Note added at 16 mins (2016-10-19 12:22:09 GMT)
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https://www.pmf.unizg.hr/_download/repository/8a_AK2_atom_sp...

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Note added at 17 mins (2016-10-19 12:23:00 GMT)
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heh, tek sam sad vidjela da sam napisala "i još dosta primjera" i onda navela jedan primjer... OMG - oprostite :-)

ipv
Native speaker of: Native in CroatianCroatian, Native in BosnianBosnian
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