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12:15 Mar 13, 2019 |
English to Polish translations [PRO] Science - Biology (-tech,-chem,micro-) | |||||||
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| Selected response from: Frank Szmulowicz, Ph. D. United States Local time: 17:07 | ||||||
Grading comment
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Summary of answers provided | ||||
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3 | analiza ilościowa obszaru ułamkowego zajmowanego przez określony typ komórki |
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analiza ilościowa obszaru ułamkowego zajmowanego przez określony typ komórki Explanation: Definicja opisowa. ccccccccccc Quantitative Analysis of Immunoreactivity Assessment of astroglia (glial fibrillary acidic protein [GFAP])and activated microglial (Human leukocyte antigen-DR [HLA-DR]) immunostaining was conducted by the unbiasedmethod of fractional area quantification as described previ-ously.25,26 The cerebral cortex of the MFG and ACG andthe granular cell layer (GCL) and white matter of the cere-bellum were outlined for quantitative analysis with the helpof a video-microscope controlled by Stereo Investigator Soft- ware (MicroBrightfield, Williston, VT). A group of 30points was systematically placed in random positions, at20m intervals, within the boundary of each region. Thesum of the points falling over structures of interest (eg, as-troglia or microglia) was divided by the total number of gridpoints sampled to estimate the fraction of the area of the region occupied by a particular type of cell. The fractional area was defined according to the Delesse principle25 as equal to the fraction of the volume occupied by the cell type being quantified. This method measures the percentage of the area of interest that is immunoreactive for a specific antibody https://www.academia.edu/10720554/Neuroglial_activation_and_... |
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