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boomerang DNA amplification

Portuguese translation: amplificação de DNA tipo bumerangue

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:boomerang DNA amplification
Portuguese translation:amplificação de DNA tipo bumerangue
Entered by: Clauwolf

03:10 Mar 5, 2019
English to Portuguese translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: boomerang DNA amplification
Non-limiting examples of nucleic acid amplification techniques include polymerase chain reaction (PCR), reverse transcriptase polymerase chain reaction (RT-PCR), nested PCR, ligase chain reaction (see Abravaya, K. et al., Nucleic Acids Res.
(1995), 23:675-682), branched DNA signal amplification (see Urdea, M. S. et al., AIDS (1993), 7(suppl 2):S11- S14), amplifiable RNA reporters, Q-beta replication, transcription-based amplification, boomerang DNA amplification, strand displacement activation, cycling probe technology, isothermal nucleic acid sequence based amplification (NASBA) (see Kievits, T. et al., J Virological Methods (1991), 35:273-286), Invader Technology, next-generation sequencing technology or other sequence replication assays or signal amplification assays.
Isadora Vital
United States
amplificação de DNA tipo bumerangue
Explanation:
:) boomerang DNA amplification (BDA)

A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373
Selected response from:

Clauwolf
Local time: 01:07
Grading comment
Selected automatically based on peer agreement.
4 KudoZ points were awarded for this answer



Summary of answers provided
3 +2amplificação de DNA tipo bumerangue
Clauwolf
4amplificação bumerangue de DNA
Monica Mazzoni


  

Answers


9 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +2
boomerang dna amplification
amplificação de DNA tipo bumerangue


Explanation:
:) boomerang DNA amplification (BDA)

A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373

Clauwolf
Local time: 01:07
Native speaker of: Native in PortuguesePortuguese
PRO pts in category: 59
Grading comment
Selected automatically based on peer agreement.

Peer comments on this answer (and responses from the answerer)
agree  Rosana de Almeida: Eu concordo também.
52 mins
  -> obrigado
agree  Margarida Ataide: ADN em PT-PT
26 days
  -> obrigado
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2 days 16 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
boomerang dna amplification
amplificação bumerangue de DNA


Explanation:
Acho que assim fica super claro que o bumerangue se refere ao método e não ao DNA.
Outra forma de referir-se ao método, mas que não cabe na frase colocada pela colega Isadora, seria método bumerangue de amplificação do DNA.
A frase de exemplo explica bem a razão para utilizar o termo boomerang.



Example sentence(s):
  • The procession of replication around the spacer 100 suggested the motion of a boomerang, hence the coined name of the present method.

    Reference: http://people.oregonstate.edu/~ahernk/bdapartII.html
Monica Mazzoni
Brazil
Local time: 01:07
Native speaker of: Native in PortuguesePortuguese
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