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03:10 Mar 5, 2019 |
English to Portuguese translations [PRO] Science - Biology (-tech,-chem,micro-) | |||||
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| Selected response from: Clauwolf Local time: 01:07 | ||||
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3 +2 | amplificação de DNA tipo bumerangue |
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4 | amplificação bumerangue de DNA |
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boomerang dna amplification amplificação de DNA tipo bumerangue Explanation: :) boomerang DNA amplification (BDA) A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373 |
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2 days 16 hrs confidence:
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