bleed-through
| 17:28 Jul 5, 2017 |
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| English to Portuguese translations [PRO] Medical - Medical: Instruments / Diagnóstico | |||||||
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| 3 | sangramento |
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| 3 | sobreposição/interferência de espectros/artefactos |
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Answers
1 hr confidence: 
sangramento Explanation: Sugestão |
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3 hrs confidence:
sobreposição/interferência de espectros/artefactos Explanation: Bleed-through occurs when you can see fluorescence from a neighboring channel in the channel of interest. This can occur in experiments that involve labeling with more than one fluorophore at a time. What you can do to minimize bleed-through •Choose your fluorophores and filters wisely. Aim to use the most suitable filter for each fluorophore you want to image. •Using fluorophores with narrow emission spectra can also help minimize bleed-through. •When designing experiments with multiple fluorophores, choose fluorophores that do not have spectral overlap. https://www.thermofisher.com/pt/en/home/life-science/cell-an... Bleed-through (often termed crossover or crosstalk) of fluorescence emission, due to the very broad bandwidths and asymmetrical spectral profiles exhibited by many of the common fluorophores, is a fundamental problem that must be addressed in both widefield and laser scanning confocal fluorescence microscopy. The phenomenon is usually manifested by the emission of one fluorophore being detected in the photomultiplier channel or through the filter combination reserved for a second fluorophore. Bleed-through artifacts often complicate the interpretation of experimental results, particularly if subcellular co-localization of fluorophores is under investigation or quantitative measurements are necessary, such as in resonance energy transfer (FRET) and photobleaching (FRAP) studies. https://www.olympus-lifescience.com/es/microscope-resource/p... La corrección de solapamiento de espectros o “bleed‐through” se realizó usando imágenes de células expresando sólo el donor (CFP) y sólo el aceptor (YFP). http://digital.bl.fcen.uba.ar/Download/Tesis/Tesis_4865_Maro... O cálculo da integral da sobreposição dos espectros do doador e aceptor foi realizado após obtenção dos espectros de absorção e de emissão de fluorescência para cada proteína de BthTx-I, e os resultados são demonstrados na Tabela 9. http://livros01.livrosgratis.com.br/cp076128.pdf Foi até onde consegui chegar, sem muitas certezas. |
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