com a visualização da expressão

English translation: expression [of peptides] was observed

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Portuguese term or phrase:com a visualização da expressão
English translation:expression [of peptides] was observed
Entered by: liz askew

18:18 Nov 17, 2011
Portuguese to English translations [PRO]
Biology (-tech,-chem,micro-)
Portuguese term or phrase: com a visualização da expressão
A expressão da proteína foi verificada em gel SDS-PAGE 15% corado com Coomassie Brilliant Blue com a visualização da expressão de peptídeos de massa molecular de aproximadamente 19KDa, fusionado a resíduos de histidina (His-Tag).
Andre Damasceno
Brazil
Local time: 17:30
expression [of peptides] were observed
Explanation:
I think you need to change the sentence round a bit

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Note added at 1 hr (2011-11-17 19:35:54 GMT)
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www.patents.com/us-20090215113.html - Translate this page
... Tris/glycine SDS-PAGE and subsequent Coomassie Brilliant Blue staining. ..... G. Peptide Production By Bacterial Expression [0097]The peptides which .... Mercaptoethanol, 12.5% Glycerol, 0.005% Bromphenol Blue) to elute bound PT. ..... Ala Ser Ala Ile 50206PRTArtificial SequenceSynthetic histidine tag 20His His ...

of course that should be


expression.......................WAS observed


depends on how you parse the English
Selected response from:

liz askew
United Kingdom
Local time: 21:30
Grading comment
3 KudoZ points were awarded for this answer



Summary of answers provided
5peptides were visualized
Christina Paiva
3 +1expression [of peptides] were observed
liz askew


  

Answers


1 hr   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +1
expression [of peptides] were observed


Explanation:
I think you need to change the sentence round a bit

--------------------------------------------------
Note added at 1 hr (2011-11-17 19:35:54 GMT)
--------------------------------------------------

www.patents.com/us-20090215113.html - Translate this page
... Tris/glycine SDS-PAGE and subsequent Coomassie Brilliant Blue staining. ..... G. Peptide Production By Bacterial Expression [0097]The peptides which .... Mercaptoethanol, 12.5% Glycerol, 0.005% Bromphenol Blue) to elute bound PT. ..... Ala Ser Ala Ile 50206PRTArtificial SequenceSynthetic histidine tag 20His His ...

of course that should be


expression.......................WAS observed


depends on how you parse the English

liz askew
United Kingdom
Local time: 21:30
Native speaker of: Native in EnglishEnglish
PRO pts in category: 66

Peer comments on this answer (and responses from the answerer)
agree  Muriel Vasconcellos: Or 'could be obeserved' or 'was seen' (I would use the singular)
3 hrs
  -> Thank you!
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5 hrs   confidence: Answerer confidence 5/5
peptides were visualized


Explanation:
Detergent binding explains anomalous SDS-PAGE migration of ...
www.pnas.org/content/106/6/1760.fullby A Rath - 2009 - Cited by 77 - Related articles
A further proviso for MW estimation by SDS-PAGE is a consistent amount of ... **Peptide segments** that contain an abundance of hydrophobic ..... ...TM3/4 hairpins [uma proteína mutante] were expressed and purified as previously described (13). ...

um trecho do artigo acima:

**Proteins were visualized** with Coomassie Blue staining. Hairpin apparent MW values were estimated from the migration rates of Mark-12 markers (Invitrogen), ..

Em eletroforese com gel de poliacrilamida aka PAGE/SDS apenas os peptídeos/proteínas são visualizados como resultado da expressão do gene/genes estudado(s)... ou seja - não se visualiza a expressão, mas seu resultado.

HTH

Christina Paiva
Brazil
Local time: 17:30
Specializes in field
Native speaker of: Native in PortuguesePortuguese, Native in EnglishEnglish
PRO pts in category: 8
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