semear alíquota de 0,1 mL
English translation: innoculate or spread evenly
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| 13:56 Aug 26, 2009 |
| Portuguese to English translations [PRO] Science - Livestock / Animal Husbandry | |||||
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|  Selected response from: Luiza Modesto Brazil Local time: 20:36 | ||||
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Answers
15 mins confidence: 
Disseminate 0,1 mL aliquots on plates containing Columbia blood agar base Explanation: My understanding of this. |
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18 mins confidence: peer agreement (net): +1
peer agreement (net): +1A 0.1 mL aliquot was PLATED on Petri dishes containing Explanation: This is how I would put it. -------------------------------------------------- Note added at 21 mins (2009-08-26 14:18:21 GMT) -------------------------------------------------- Semear (to plate). É o mesmo que fazer uma cultura. Alternativa: 0.1 mL aliquots were CULTURED on plates containing... Example sentence(s):
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1 hr confidence: peer agreement (net): +1
peer agreement (net): +1innoculate or spread evenly Explanation: The first step is to inoculate the petri dish with as diluted a mixture as possible. The books are full of procedures for doing this but I find the simplest is just as good. Take a copper wire or thin glass rod and heat several inches in a flame to sterilize. Dip this, when cool, into a working beer or yeast culture. If starting with dry yeast, dissolve one granule of yeast in a test tube with about one inch of sterile water. Gently drag the inoculated wire across the gelatin in the petri dish, trying not to break the surface. http://brewery.org/brewery/library/yeast_arf.html Turn the Petri dish right side up, • Open the unknown culture tube, and flame its opening, • Open the lid of the Petri dish only part way: just enough so that you can pour the unknown on the agar surface; make a puddle a little smaller than the size of a dime, • Close the Petri dish, • Flame the opening of the unknown culture tube and close it, then • Take the glass elbow (called a spreader) from its container of alcohol, tapping as much alcohol as possible off its surface against the inside wall of its container, • Carefully flame the elbow and hold it until it cools slightly, • Open the Petri dish just enough to admit the glass elbow, • Use the sterile spreader to ****spread the food solution evenly**** over the surface of the Petri dish, • Close the Petri dish, • Reflame the glass elbow, let it cool, and return it to the alcohol solution, • Secure the Petri dish with several pieces of tape and • Place upside-down (agar hanging) Petri dish in incubator. Reference: http://www.hccfl.edu/media/33304/1011_lab_04_preparation_of_... |
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