inoculum pooled cell culture
Portuguese translation: produção de inóculo a partir de cultura concentrada de células
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| 23:42 Sep 25, 2008 |
| English to Portuguese translations [PRO] Medical: Pharmaceuticals / processo de validação | |||||||
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|  Selected response from: Maria Teresa Borges de Almeida Portugal Local time: 22:13 | ||||||
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| 4 +2 | produção de inóculo a partir de cultura concentrada de células |
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| 4 | cultura de células em inoculum agrupado |
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Answers
1 hr confidence: 
cultura de células em inoculum agrupado Explanation: Agrupado em frascos, separadamente. por exemplo. inoculum = substância utilizada na inoculação -------------------------------------------------- Note added at 1 hr (2008-09-26 01:29:34 GMT) -------------------------------------------------- Disposable perfusion bioreactor for cell culture - Patent 6544788 A disposable bioreactor for perfusion cell culture. ... Cellbag® 2L bag bioreactors were run simultaneously using inoculum pooled from five T-175 flasks. ... www.freepatentsonline.com/6544788.html - |
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7 hrs confidence: peer agreement (net): +2
peer agreement (net): +2produção de inóculo a partir de cultura concentrada de células Explanation: Diria assim... Example sentence(s):
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| Reference Reference information: Christina, não se se vai ajudar muito, mas eu acho que isto é pelo menos uma definição do processo em questão: "Generation of hybridomas For more details on this topic, see Hybridoma. It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymphocytes isolated from the spleen (or possibly blood) of an immunised animal are combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specifity of the primary lymphoctye and the immortality of the myleoma. Selective growth medium (HA or HAT) is used to select against unfused myeloma cells; primary lymphoctyes die quickly in culture and only the fused cells survive. These are screened for production of the required antibody, **generally in pools to start with and then after single cloning**." Ou seja, a tal cultura celular "pooled" é um estágio final da produção dessas células híbridas que funcionam como anticorpos mas que são imortais. É de se presumir que o "inoculum" no caso seja, então, um anticorpo ou algo parecido. Se for isso mesmo, uma tradução meio "crua" poderia ser "cultura celular agrupada do inoculante". Mas isso precisaria ser melhorado... Reference: http://en.wikipedia.org/wiki/Cell_culture#Generation_of_hybr... |
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