Glucose spike

Russian translation: добавка глюкозы

16:14 Nov 29, 2007
English to Russian translations [PRO]
Medical - Medical: Pharmaceuticals / immunology
English term or phrase: Glucose spike
Preparation of Hemophilus influenza type b vaccine.
Stage 2
Fermentation of Hib is done in fermentor with 30L capacity and working volume 20-24L. Fermentor is sterilized at 121 C for 30 minutes with Basal medium. Supplements are added by filter sterilization. The seed is used to inoculate the fermentor under aseptic conditions. Cultivation is performed at temperature 34-36 with agitation of 300-600 RPM, DO set point 20%, pH 7,1 for 8-12 hours with Glucose spike 1-2 of 20% each. OD590 is monitored on spectrophotometer. When it is >3,5 and stationary phase has reached, purity of broth is checked.
Olena Kushnerenko
Ukraine
Local time: 05:02
Russian translation:добавка глюкозы
Explanation:
Пример: In the repeated fed-batch studies, reactors were spiked periodically with concentrated glucose (75%) to maintain a concentration of ~80 g of glucose l-1 in the bioreactor.
http://www.springerlink.com/content/et27h2gf3dpp1ga8/
Selected response from:

Pavel Venediktov
Russian Federation
Local time: 06:02
Grading comment
4 KudoZ points were awarded for this answer



Summary of answers provided
5 +4добавка глюкозы
Pavel Venediktov
4нагрузка глюкозой
Marina Mrouga


  

Answers


10 mins   confidence: Answerer confidence 5/5 peer agreement (net): +4
glucose spike
добавка глюкозы


Explanation:
Пример: In the repeated fed-batch studies, reactors were spiked periodically with concentrated glucose (75%) to maintain a concentration of ~80 g of glucose l-1 in the bioreactor.
http://www.springerlink.com/content/et27h2gf3dpp1ga8/

Pavel Venediktov
Russian Federation
Local time: 06:02
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1149

Peer comments on this answer (and responses from the answerer)
agree  Leila Usmanova
19 mins

agree  Mikhail Yanchenko
28 mins

agree  cherepanov
2 hrs

agree  Natalie
3 hrs
Login to enter a peer comment (or grade)

40 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
glucose spike
нагрузка глюкозой


Explanation:
по аналогии с пробой с нагрузкой глюкозой, цель которой вызвать резкий скачок концентрации глюкозы в крови (glucose spike)

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Note added at 46 mins (2007-11-29 17:00:49 GMT)
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В этом фрагменте видно, что glucose spike в 10 раз больше, чем добавление глюкозы в обычном режиме. Поэтому можно предложить и другой перевод - "введение глюкозы ВЫСОКОЙ КОНЦЕНТРАЦИИ"

Mapping Stress-Induced Changes in Autoinducer AI-2 Production in ...We next perturbed steady-state W3110/phIL-2 cultures grown in LB medium–5 mM glucose with a glucose spike (50 mM pulse fed to reactor). ...
www.pubmedcentral.nih.gov/articlerender.fcgi?&pubmedid=1129...


Marina Mrouga
Local time: 06:02
Specializes in field
Native speaker of: Russian
PRO pts in category: 270
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