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  3. Biology (-tech,-chem,micro-)

spiked blank recovery

English translation: spiked blank recovery

17:21 Aug 8, 2006
English language (monolingual) [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: spiked blank recovery
I need a good (i.e. comprehensive to me) explanation of this meat safety testing method. Moreover, I would like to know how this is different from **spiked recovery" also seen in the following sentence.
Following is the context:

As part of the quality control, measurements of the standards (spiked blank recovery) to validate the chromatographic method and measurements of recovery of each analyte from a spiked sample (spiked recovery) were included.
humbird
Selected answer:spiked blank recovery
Explanation:
Apparently, the amounts of one or more substances in meat samples are being determined by a method involving extraction and chromatography. As extraction is unlikely to extract 100% of the substance(s), a known amount of a similar substace is added to the sample before extraction (this is the spike). The amount of this substance detected on the chromatogram divided by the original amount added gives the recovery, expressed as a % (= spike recovery).
In order to know the amount of substance represented by the chromatography peaks, standards, i.e. solutions containing a range of known amounts of the substance(s) being measured, are processed in the same way as the meat samples (extraction + chromatography). As these standards contain no meat sample material, they are "blanks". Recovery must also be determined for these blank standards, so they are "spiked" by addition of the same spike substance as the meat samples (=> spike blank recovery).
Note: This does not seem to be the usual meaning of "blank", which is a test on a solution containing none of the substance being determined.
Selected response from:

David Sirett
Local time: 20:33
Grading comment
Thank you for your detailed and comprehensive answer. This makes sense.
4 KudoZ points were awarded for this answer



SUMMARY OF ALL EXPLANATIONS PROVIDED
4 +3spiked blank recovery
David Sirett


  

Answers


13 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +3
spiked blank recovery


Explanation:
Apparently, the amounts of one or more substances in meat samples are being determined by a method involving extraction and chromatography. As extraction is unlikely to extract 100% of the substance(s), a known amount of a similar substace is added to the sample before extraction (this is the spike). The amount of this substance detected on the chromatogram divided by the original amount added gives the recovery, expressed as a % (= spike recovery).
In order to know the amount of substance represented by the chromatography peaks, standards, i.e. solutions containing a range of known amounts of the substance(s) being measured, are processed in the same way as the meat samples (extraction + chromatography). As these standards contain no meat sample material, they are "blanks". Recovery must also be determined for these blank standards, so they are "spiked" by addition of the same spike substance as the meat samples (=> spike blank recovery).
Note: This does not seem to be the usual meaning of "blank", which is a test on a solution containing none of the substance being determined.

David Sirett
Local time: 20:33
Specializes in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 24
Grading comment
Thank you for your detailed and comprehensive answer. This makes sense.

Peer comments on this answer (and responses from the answerer)
agree  Uma Hariharan
3 hrs
agree  Ana L Fazio-Kroll
9 hrs
agree  Alfa Trans (X)
12 hrs
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